To evaluate the risk of disease development, such as cancers and lifestyle-related diseases, individual differences of a single nucleotide in genes (single nucleotide polymorphism, SNP) are generally analyzed. However, sensors based on conventional systems may misrecognize genes other than the disease-related genes depending on the target sequences used. We are developing new DNA sensors based on the interaction between solution and DNAs to increase the recognition ability of a target gene by reduction of such misrecognition.
Reference
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H. Tateishi-Karimata, S. Pramanik, and N. Sugimoto
DNA sensor’s selectivity enhancement and protection from contaminating nuclease due to hydrated ionic liquid
Analyst, 140, 4393-4398 (2015) [Selected as a Front Cover] -
T. Endoh and N. Sugimoto
Rational design and tuning of functional RNA switch to control an allosteric intermolecular interaction
Anal. Chem., 87, 7628-7635 (2015) -
T. Endoh and N. Sugimoto
Aptamer-based universal fluorometric sensors based on allosteric modulation of RNA–peptide Interactions
ChemMedChem, 9, 2045-2048 (2014) -
T. Endoh and N. Sugimoto
Selection of RNAs for constructing “Lighting-UP” biomolecular switches in response to specific small molecules
PLoS ONE, 8, e60222 (2013)
Reviews and books
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遠藤玉樹, 杉本直己
細胞内核酸化学
ここまで進んだバイオセンシング・イメージング 1分子から細胞，脳まで
CSJカレントレビュー（化学同人）, 86-91 (2012)
Others
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杉本直己, 遠藤玉樹
RNAとタンパク質で発光する分子センサーの開発
工業材料（日刊工業出版プロダクション）61(12), 22-25 (2014)